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phase sandwich method  (R&D Systems)


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    R&D Systems phase sandwich method
    Phase Sandwich Method, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+method/pmc12974880-98-15-20?v=R%26D+Systems
    Average 96 stars, based on 267 article reviews
    phase sandwich method - by Bioz Stars, 2026-07
    96/100 stars

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    CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

    Article Snippet: The concentration of Angptl4 was determined via the ELISA method (CUSABIO Co., Ltd., Wuhan, China, cat# CSB-EL001712MO).

    Techniques: Cell Function Assay, Tube Formation Assay, Wound Healing Assay, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay